Thp1 assay
WebIn addition, The transwell assay was conducted by adding macrophages to the upper chamber. After 24 h, The number of macrophages in the CCL5-treated group increased significantly (Figure 5A and B), indicating that CCL5 … WebTHP1-Blue™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible SEAP reporter construct. THP1- Blue™ NF-κB cells express a secreted …
Thp1 assay
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WebBelow are a few tips we recommend to help get your THP1 cells growing: • These cells need to be passaged between 3 x 10 5 and 7 x 10 5 cells/ml (we recommend 5 x 10 5 cells/ml). … WebApr 11, 2024 · Using a 24-well plate, a cellular density of 3 × 10 5 cells/well/ml growth medium was used for the binding assays. THP1 cells were grown on 6-well plates coated with 8 nM phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich, St. Louis, MO, USA) at a density of 5 × 10 5 cells/well/2 ml growth medium for RT‒qPCR, and for fluorescent ICC …
WebJun 1, 2024 · In my hands, THP1 do not express CD14 and when treated with PMA (10, 50 and 100 ng/ml) the percentage of expression increases, being greater at 10 ng/ml. In … WebMultiTox-Fluor Multiplex Cytotoxicity assays were performed using four increasing concentrations of DUBs-IN2 inhibitor in THP-1 macrophages as indicated on the figures. Cells were treated with either vehicle control or DUBs-IN2 for either a 2- or 24-hour period, after which the assay buffers were added and incubated for 30 minutes before …
WebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase reporter cell line was derived from parental line TIB-202 by stably expressing firefly luciferase gene (luc2) under control of a NF-κB promoter. This cell line was established … WebAug 7, 2008 · 160 nM PMA (Sigma) in RPMI medium was added to differentiate the THP-1 cells for 72 h at 37°C for all the cytokine assays through this study. The criteria for the differentiation of the THP-1 cells, adherence, morphological changes, and changes in expression of the cell surface makers such as integrin, FcγRI, CD4, and MHC class II …
WebTable 1. EC 50 values derived from a 4-parameter fit using SoftMax Pro Software.. Conclusion. We present a multiparametric inflammation assay that uses a THP-1 cell model with the ImageXpress Pico—an automated cellular imaging system—the Pu·MA System—a novel, low-volume automated ELISA system—and the SpectraMax iD5 microplate reader.
WebApr 12, 2024 · An MTT assay was used to examine cell viability. Apoptosis was detected using FITC (fluorescein isothiocyanate) annexin V apoptosis detection kit. Western blotting was used to separate and identify proteins. ... Calcitriol also inhibited CCM-mediated induction of CD206 and increased TNFα gene expression in THP1-derived macrophages. roswell do it center roswell nmWebJan 13, 2024 · Allergen recognition and processing by antigen presenting cells is essential for the sensitization step of food allergy. Macrophages and dendritic cells are both phagocytic antigen presenting cells and play important roles in innate immune responses and signaling between the innate and adaptive immune system. To obtain a model … story of the childrenWebDec 31, 2024 · For in vitro osteoclast differentiation assays, RAW264.7 cells were seeded at a density of 25,000 cells per well in 24‐well tissue culture ... NF‐κB reporter assay. THP1‐Dual cells were maintained in culture medium containing 10 µg/ml blasticidin and 100 µg/ml Zeocin to maintain selection pressure and passaged every three ... roswell dodge dealershipWebNF-kB-SEAP and IRF-Lucia luciferase Reporter Monocytes. THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter … roswell donut shop roswellAlthough THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their characteristics are: • Expression of Fc receptor and C3b receptors while lacking surface and cytoplasmic immunoglob… story of the chinese zodiacWebJun 21, 2024 · We used our previously established FACS-based phagocytosis assay 20 and performed a genome-wide CRISPR screen in THP-1 cells phagocytosing S. aureus particles. Subsequently, ... story of the chinese farmerWebComparative genotoxicity evaluations were made with H2 O2 or Paraquat treated TPH-1 cells. Comet assays were performed at 1 h (4°C); after a 24-h recovery period (37°C); and … roswell documentary filing cabinet