Phospho p53bp1 fluorescent staining
WebImmunofluorescence staining was performed as described previously . Antibodies against 53BP1, Chk2, Chk2T68P, and γ-H2AX were generated as described previously ( 22 , 26 , 27 … WebPhospho-53BP1 (Ser1778) Antibody detects endogenous levels of 53BP1 only when phosphorylated at serine 1778. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic …
Phospho p53bp1 fluorescent staining
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Web53BP1 was originally identified as a binding partner for p53, and suggested to enhance its transcriptional activity 6, 7. 53BP1 plays an essential role in DNA repair; it is known to be recruited to sites of DNA damage, and retention of 53BP1 at these sites is dependent on gamma-H2A.X 8. WebSep 17, 2003 · In order to optimize staining levels, phospho-specific mAbs were titrated on unstimulated and stimulated samples to find the concentration that maximized the fold …
WebPhospho-53BP1 (Thr543) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan ®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Thr543 was discovered using an ATM/ATR substrate antibody and was shown to be induced by UV treatment. WebFluorescence Protection Program: Extend the Lifetime of Your Fluorescent Antibodies and Reagents. Fluorescent reagents may require special storage and handling, to maintain the …
WebThe staining patterns of 53BP1 nuclear foci and 53BP1 mRNA level are associated with cervical cancer progression and metastasis. p53-binding protein 1 (53BP1) plays a key …
WebResuspend the cells in Stain Buffer (FBS) at 10 million cells/mL and aliquot 100 μL to each sample tube. Aliquot an optimal concentration of phosphospecific fluorochrome …
WebFigure 1. Phospho-specific Antibody Arrays Referenced in Primary Literature. There are over 200 references to phospho-specific, membrane-based antibody arrays in the primary … high rocks pub gladstoneWebWestern blot analysis of extracts from Jurkat cells, untreated (-) or treated with LY294002 #9901 or Calyculin A #9902 (+), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060 (upper), Akt (pan) (C67E7) Rabbit mAb #4691 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). The western blot membranes were treated with our standard antibody diluent and … high rocks park portlandWebOct 17, 2024 · These findings suggests that ginsenoside Rg3 regulates VRK1 signaling. Immunofluorescence assays showed that P53BP1 and VRK1 protein level increased, and the VRK1 protein translocated between the... how many carbs in 1 oz of sweet potatoWebPhospho-p53 (Ser15) Antibody detects endogenous levels of p53 only when phosphorylated at serine 15. The antibody does not cross-react with p53 phosphorylated at other sites. … how many carbs in 1 oz pepper jack cheeseWebCheck the product information for recommendations for each primary antibody being used. Preheat the antigen retrieval buffer (100 mM Tris, 5% [w/v] urea, pH 9.5) to 95°C. This can … how many carbs in 1 oz of whiskeyWebFluorochrome-conjugated antibodies can be used to stain surface proteins for the purpose of immunophenotyping cells that will be further analyzed for phosphorylated proteins; … how many carbs in 1 slice of american cheeseWebA fluorescent staining procedure has been developed which rapidly, accurately, and economically measures the viability of mycobacterial cells. M. smegmatis and M. phlei have served as prototype organisms to establish conditions which ensure optimal staining. The staining method incorporates the use of the fatty acid ester fluorescein diacetate ... high rocks pub gladstone oregon