Cytoplasmic asters

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August undefined, 2024

WebIn a number of studies Taxol has been reported to induce cytoplasmic MTs asters in cells treated with the drug [e.g., [9,10,28, 29]]. Most of these studies have used Taxol concentrations above 0. ...

Cytoplasmic Asters Are Required for Progression Past the First …

WebMEK (MAPK kinase) is an upstream protein kinase of MAPK in the MOS/MEK/MAPK/p90rsk signaling pathway. We previously reported the function and regulation of MAPK during rat oocyte maturation. In this study, we further investigated the localization and possible roles of … WebDownload scientific diagram Fertilization in F. fimetaria eggs ( green microtubules, red DNA). a The sperm aster ( arrow ) is visible even in the oocyte cytoplasm during late metaphase ( large ... greek god of craftsmanship

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WebMay 10, 2002 · The bacterium Wolbachia manipulates reproduction in millions of insects worldwide; the most common effect is cytoplasmic incompatibility (CI). We found that CI … WebMost insects have a single NP gene and multiple NPs and NP-like peptides are produced by extensive alternative splicing 3, 5 or the different processing of the precursor peptide. 2 Alternatively, various decapods 4 and several hemipteran insects 6, … WebAug 15, 2003 · Tubulin staining reveals that the asters assemble spontaneously only in the anterior region of the parthenogenetic egg, whereas the remaining cytoplasm is devoid of these structures (Fig. 1F–H).We found only two exceptional eggs in which the asters form through the whole cytoplasm among all the parthenogenetic eggs scored (n = … greek god of craft

Gradual Meiosis-To-Mitosis Transition in the Early Mouse Embryo

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WebNov 11, 2011 · However, recent work has shown that they do assemble multiple acentriolar MTOCs prior to NEB. 68 On average, ∼80 MTOCs or asters form in each oocytes; these form both out in the cytoplasm and along the nuclear surface. The cytoplasmic asters were observed to migrate toward the nuclear region, and both cytoplasmic and nuclear … WebThe cytoplasmic foci were larger and more abundant than in untreated oocytes (Table 1). Double staining revealed that the anti-γ- tubulin reactivity was found in the center of all the asters... greek god of conflictWebThe cytoplasmic asters became undetectable within 2 h of oocyte activation, irrespective of the presence or absence of the donor chromosomes. After the standard NT protocol, a … flow cmc

"WebNov 1, 2003 · So the cytoplasmic asters as well as nucleation of microtubules from the spindle poles is solely due to DOC1R depletion. Altogether, our experiments demonstrate that DOC1R regulates microtubule organization at least in metaphase II (see Discussion). The depletion of DOC1R induces severe damage to the microtubule cytoskeleton, which … " - Cytoplasmic asters

Cytoplasmic asters

WebSep 1, 1998 · These unique results not only provide a demonstration that cytoplasmic dynein is required for the formation of mitotic asters in N. haematococca; they also represent the first report of mitotic phenotypes in a dynein mutant of any filamentous fungus and the first cytoplasmic dynein mutant of any organism whose mitotic phenotypes … WebMar 15, 2012 · In the next experiment, multiple asters observed in 5.5 vs 8 hpi pronuclear zygotes were located near the male pronucleus, suggesting that those multiple asters were not the cytoplasmic asters of maternal origin. In conclusion, multiple aster formation frequently observed in vitrified-warmed bovine oocytes may be related to loss of …

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WebDec 1, 2011 · Abstract. Cytasters (cytoplasmic asters) are centriole-based nucleation centers of microtubule polymerization that are observable in large numbers in the … WebJan 1, 2005 · The cytoplasmic asters became undetectable within 2 h of oocyte activation, irrespective of the presence or absence of the donor chromosomes. After the standard NT protocol, a spindle-like ...

WebJan 1, 2005 · Cytoplasmic asters were localized by staining with an anti-alpha-tubulin antibody. Enucleation of MII oocytes caused no significant change in the number of … WebJan 23, 2012 · Of the 233 embryos belonging to the syncytial blastoderm mitoses, 61% (n = 142) progressed normally through the nuclear division cycles, and 39% (n = 91) showed a wide range of mitotic defects. Defects in chromosome …

WebRemarkably, the asters have been observed only in the anterior cytoplasm of the parthenogenetic eggs (Riparbelli and Callaini, 2003; Fig. 8 C and D). Aster formation presumably involves the overriding of intrinsic control points that prevent centrosome self-assembly in dioic eggs, thus avoiding attempt to develop in the absence of a male gamete. WebApr 15, 2024 · The sperm aster is situated in the inner cytoplasm and its MTs are connected to the parallel MTs in the shear zone (Houliston and Elinson, 1991a). The cortex region consists of a plasma membrane, actin cytoskeletal filaments, and the endoplasmic reticulum (ER) ( Houliston and Elinson, 1991a ).

WebUnlike the oocytes of most other animal species, unfertilized murine oocytes contain cytoplasmic asters, which act as microtubule-organizing centers following fertilization. This study examined the role of asters during the first cell cycle of mouse nuclear transfer (NT) embryos. NT was performed by intracytoplasmic injection of cumulus cells. Cytoplasmic …

WebRemarkably, the asters have been observed only in the anterior cytoplasm of the parthenogenetic eggs (Riparbelli and Callaini, 2003; Fig. 8 C and D). Aster formation … flow club valenciaWebDec 7, 2024 · We observed all cytoplasmic networks mostly moved together inside asters. The highest velocity differences between networks occurred at the aster periphery. Mechanical integration between all … greek god of creative skillWebSep 1, 1998 · These unique results not only provide a demonstration that cytoplasmic dynein is required for the formation of mitotic asters in N. haematococca; they also … flow club at truist stadiumWebnumerous astral microtubules (cytoplasmic asters) in the mature M2 oocyte cytoplasm. An astral microtubule aids in the male and female genome union during fertilization. In mouse oocytes, several pericentriolar material foci have been found in acentriolar astral microtubules (cytoplasmic asters) [10]. It is thought that the MTOCs of cytoplasmic ... flow club costWebJan 1, 2012 · The cores of these asters are composed of a few PCM components, including pericentrin and gamma-tubulin. Based on this composition and the ability of these … flow clusteringWebDec 1, 2004 · Cytoplasmic asters persist in the cytoplasm (arrows) and around the donor nucleus (arrowheads). At 8–12 h (B 2), a large pseudopronucleus develops and asters … flow club pathankotWebFeb 22, 2024 · Asters are radial microtubule arrays found in animal cells. These star-shaped structures form around each pair of centrioles … greek god of crafting