Cy3 goat anti-rabbit igg
WebCy3, Goat Anti-Rabbit IgG Cy3, Goat Anti-Rabbit IgG . Views (797) Publications (20) Catalog no (A22220) Datasheet Print Share Email Share $ 29 $ Size and Unit. Cy3, Goat Anti-Rabbit IgG quantity. Add to cart Buy Now. Online Service Inquiry. Specification; FAQ; Publications(20) Comments(0) Specification. Product name ... WebDetection: ECL Enhanced Kit (RM00021). Exposure time: 45s. Western blot analysis of extracts from wild type (WT) and KRAS Rabbit mAb knockout (KO) 293T (KO) cells, using KRAS Rabbit mAb (A23382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Cy3 goat anti-rabbit igg
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WebWhole IgG Affinity-Purified Secondary Antibodies. Whole IgG antibodies are isolated as intact molecules from antisera by affinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds (Figure 1), and therefore are divalent. The average molecular weight is reported to be about 160 kD. WebCross-reactivity of Goat anti-Rabbit IgG H&L and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT.
WebSuggested dilution for most applications: 1:100-1:800 for most applications. Optimal working dilutions must be determined by the end user. This Goat anti-Rabbit IgG Antibody, Cy3 conjugate, Species Adsorbed is validated for use in IF for the detection of Rabbit IgG. WebSecondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody used at 1/40000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa …
WebCy3 is brighter, more photostable, and gives less background than other orange-red fluorescing dye conjugates. ... Connexin 26 (blue, Alexa532-conjugated anti-goat IgG), … WebAnti-Rabbit IgG (whole molecule), F (ab′)2 fragment–Cy3 antibody produced in sheep. affinity isolated antibody, buffered aqueous solution. View Price and Availability. Sigma …
WebBlue: DAPI for nuclear staining. Western blot analysis of HeLa, using EGFR Rabbit mAb (A23381) at 1:2500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
WebWe offer Anti-Mouse secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Rabbit, Rat and Sheep. JIR secondary antibodies are available as whole IgG and F (ab') 2 fragment formats. Fab fragment formats are also available and can be an elegant component of multiple blocking and labeling protocols. does prednisolone make u sleepyWebServicebio Inc cy3 conjugated goat anti rabbit igg secondary antibody Cy3 Conjugated Goat Anti Rabbit Igg Secondary Antibody, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more does povidone iodine stain skinWebGoat Anti-Mouse IgG Antibody, Cy3 conjugate Chemicon®, from goat; find Sigma-Aldrich-AP124C MSDS, related peer-reviewed papers, technical documents, similar products & … does prednisone make you urinateWebSep 24, 2012 · Specificity. By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit … does prednisolone make you tiredWebThe cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007). Blue: DAPI for nuclear staining. … does prednisone raise blood sugarWebWestern blot analysis of various lysates, using RELB Rabbit mAb (A23389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. does prednisone make you tiredWebFor flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA. does prednisone make you shake