Chromium single cell 3' reagent kit v2
WebOverview. 10X Genomics 3’ Gene Expression. The 10X Genomics Chromium Single Cell 3’ GEM Library and Gel Bead Kit v3 enables simultaneous library preparation of hundreds to thousands of individual cells for 3’ digital gene expression profiling analysis. Cell suspensions (containing 800 to 16,000 cells per sample) are loaded into a Chromium ... WebGitHub Pages
Chromium single cell 3' reagent kit v2
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WebFor RNA sequencing, samples were prepared using the Chromium Single Cell 5’ Library & Gel Bead Kit v2 (10X Genomics) according to the manufacturer’s protocol with a capture target of 10,000 cells. ... (2.0.3) algorithm(71) using the multiplet rates in the 10X Genomics Chromium Single Cell 3′ Reagent Kits User Guide (v3 Chemistry ... WebChromium Single Cell Reagents With our reagent kits, capture and amplify mRNA, profile the immune repertoire, identify CRISPR perturbations, detect chromatin accessibility, and more. Analysis and …
WebApr 14, 2024 · BM mononuclear cells (BMMC) were isolated using Ficoll-Paque purification and cryopreserved in a 1:10 mixture of dimethyl sulfoxide and fetal bovine serum. Upon thawing in 37°C water baths, whole BMMCs from WashU Cohort 1 were loaded onto the 10X Genomics Chromium Controller using 10X Genomics Chromium Single-Cell 3′v2 … WebJun 18, 2024 · Chromium Single Cell 3’ Reagent Kits v2 User Guide • Rev D to Rev E Revision Summary 2 . Step . Page (in Rev E) Change . 2.4 a . 27 . Updated: a) Run 1 μl of sample at a dilution of 1 part sample:5 parts nuclease-free …
WebChromium Single Cell 3’ Reagent Kits v2 v Chromium Accessories viii Additional Kits, Reagents & Equipment ix Recommended Thermal Cyclers xi. The Single Cell 3’ … WebFeb 1, 2024 · Complementary DNA (cDNA) libraries of CMV-Tetramer+ CD8+ T cells were generated using the Chromium Single Cell 3′ Reagent Kits v2 while CMV-Tetramer− CD8+ T cells and CD8+ stimulated T cells were generated using the Chromium Single Cell 5′ Reagent Kits v1 with Human T cell V(D)J enrichment kits (10× Genomics). The …
WebChromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for CRISPR Screening ; Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for Cell Surface Protein; Chromium Single Cell 3' v2 Reagent Kit, Quick Reference Cards (v2 Chemistry)
WebChromium™ Single Cell 3’ Reagent Kits v2 User Guide • Rev C 10 Click to TOC Cell Suspension Volume Calculator Table Consult the Single Cell Protocols Cell Preparation Guide and Guidelines for Optimal Sample Preparation flowchart (Documents CG00053 and CG000126) for more information on preparing cells for use in this Protocol. iowa coon seasonWebCurrent Weather. 11:19 AM. 47° F. RealFeel® 40°. RealFeel Shade™ 38°. Air Quality Excellent. Wind ENE 10 mph. Wind Gusts 15 mph. oosh activitiesWebMiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications. Get faster cycle times, longer reads and more output from improved chemistry. Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit. Extend your read lengths with the 500-cycle kit. iowa coordinatesWebApr 12, 2024 · Single-cell library preparation and sequencing. Single-cell libraries were constructed using the 10X Single cell 3’ Library & Gel Bead Kit v3 according to the manufacturer’s protocol. 67 In short, the cell counts were assessed by a hemocytometer (Luna-FL, Logos Biosystems) and the cell concentration was adjusted to 1000 cells/μL. … iowa cool placesWebChromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) User Guide with Feature Barcoding technology for Cell Surface Protein (CG000317) ... Chromium Next GEM Single Cell 5' HT Reagent Kits v2 with Feature Barcode technology for Cell Surface Protein (CG000424) About. Solutions; Technology; Company; Careers; Support; … oosha contact numberWebChromium™ Single Cell 3’ Reagent Kits Quick Reference Cards FOR USE WITH Chromium™ Single Cell 3' Library, Gel Bead & Multiplex Kit, 16 rxns PN-120243 Chromium™ Single Cell 3’ Chip Kit PN-120242 CG00037 Rev A 1.1 Cell Harvesting - Suspension Cell Lines – Determine the cell concentration. Ideally, 3 x 105– 1 x 106/ml … iowa controlled substanceWebChromium Single Cell 3′ Library & Gel Bead Kit v2, Chromium: Cat#PN-120247 ... Further information and requests for resources and reagents should be directed to and will be ... PC-9 cells were trypsinized into single-cell suspensions and processed using Chromium Single Cell Gene Expression 3′ Library and Gel Bead Kit V2.0 following the ... oosh activities for children